Description
Product Overview BEGM® Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGM® BulletKit® is serum-free and has been optimized to grow cells from normal and diseased tissues. Published research shows BEGM® BulletKit® has expanded applications. Our customers have used BEGM® BulletKit® to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the 'Data' tab. BEGM® is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGM® Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGM® BulletKit® Media may also offer cost-benefit since customers can use the same media to support multiple cell types. Here are publications out of many where our customers have used BEGM® Growth Media with normal bronchial epithelial cells: El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGM® Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells Hagiwara, et al. (2015). Nature demonstrates BEGM® Media can ideally support co-culture of Lonza's HUVEC and NHBE in a 3D environment. Rider, et al. (2015). PLoS ONE 10(1) used BEGM® Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs Request More Info
Storage and Content 1 x BEBM® Basal Medium (CC-3171), 500 mL 1 x BEGM® SingleQuots® Supplement Pack (CC-4175) containing: 1 x Orange Cap Vial with BPE, 2.00 mL 1 x Lilac Cap Vial with Insulin, 0.50 mL 1 x Natural Cap Vial with Hydrocortisone, 0.50 mL 1 x Red Cap Vial with GA-1000, 0.50 mL 1 x Amber Vial with Retinoic Acid, 0.50 mL 1 x Natural Cap Vial with Transferrin, 0.50 mL 1 x Amber Vial with Triiodothyronine, 0.50 mL 1 x Amber Vial with Epinephrine, 0.50 mL 1 x Green Cap Vial with hEGF, 0.50 mL Download our Airway Cell Culture Media Concentration Guide
