Description
Product Overview The OsteoImage TM Mineralization Assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralization. The OsteoImage TM Assay can quantitate in vitro mineralization by osteogenic stem cells, primary osteoblasts, and osteoblast-like cell lines. It is based on specific binding of the fluorescent OsteoImage TM Staining Reagent to the hydroxyapatite portion of bone-like nodules deposited by cells. Unlike typical histochemical methods such as von Kossa and Alizarin red, neither of which is hydroxyapatite specific, the OsteoImage TM Assay eliminates multiple steps or tedious extraction steps. Increase the speed, sensitivity and ease of measuring mineralization in your cell cultures with the OsteoImage TM Mineralization Assay. Request More Info
Benefits Delivers qualitative visual fluorescent microscopy or quantitative fluorescent plate reader results Can be used with primary osteoblasts, osteoblast stem cells, and osteoblast cell lines Measures hydroxyapatite, similar to real bone Completed in less than 90 minutes, without tedious extractions Sensitive enough to detect time dependent increases in mineralization in differentiating cells Scalable for use in 6-well to 96-well plates
Storage and Content 5 x 96 well plates 1 x 500 μl OsteoImage TM Staining Reagent 1 x 50 mL Staining Reagent Dilution Buffer 1 x 50 mL OsteoImage TM Wash Buffer (10x)
