Description
Product Overview Stem cells possess the unique ability to differentiate into multiple lineages of cells including chondrocytes. Lonza’s hMSC Chondrogenic Differentiation BulletKit® Medium is a serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin. Partial list of cells differentiated with hMSC Chondrogenic Differentiation BulletKit® Medium*: Human bone marrow derived stem cells Human umbilical cord blood derived stem cells Human umbilical cord matrix derived stem cells C57BL/6 mice bone marrow derived stem cells Human adipose derived stem cells Human hamstring tendon derived stem cells The hMSC Chondrogenic Differentiation BulletKit TM Medium, with the addition of TGF-β3 (sold separately) provides a differentiation medium guaranteed to induce chondrogenic differentiation of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes. Each bottle of the hMSC Chondrogenic Differentiation BulletKit® Medium contains enough differentiation medium to fully differentiate at least 2 million human bone marrow derived mesenchymal stem cells (approximately 4 complete 24-well plates) into mature, functionally active chondrocytes. The hMSC Chondrogenic Differentiation Medium is offered as a BulletKit® Medium (catalog no. PT-3003) which includes both the basal media and the necessary supplements (with the addition of TGF-β3 [sold separately, catalog no. PT-4124]) for chondrogenic differentiation of human bone marrow derived mesenchymal stem cells. The basal medium (Differentiation Basal Medium-Chondrogenic; catalog no. PT-3925) and the necessary supplements (HMSC SQ Kit-Chondro; catalog no. PT-4121 and TGF Beta 3 for HMSC Chondro diff; catalog no. PT-4124) can also be purchased individually. Lonza guarantees the performance of Clonetics® /Poietics® cells only if appropriate Clonetics® /Poietics® media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support . * List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells. Request More Info
