Description
Product Overview The SF Cell Line 4D-Nucleofector TM LV Kit L* is suited for larger scale transfection of cell lines in the 1 mL Nucleocuvette TM Cartridge format when working with the 4D-Nucleofector TM LV Unit. With the 1 mL Nucleocuvette TM Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port. This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal Nucleofector TM Solution: HEK293 suspension clones CHO-S suspension clones K-562 U-937 The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. *Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-Nucleofector TM System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support. Request More Info
Benefits Closed Nucleofection of up to 10 8 cells Conditions transferable from existing smaller scale protocols Simple manual filling via sterile injection port Transfection with seconds Applications For large scale transfection of up to 1 x 10 8 cells Transient production of potential therapeutic proteins or antibodies for construct screening Generation of large numbers of transiently modified cell lines for cell-based assays
Storage and Content 2.25 mL SF Cell Line Nucleofector TM Solution 0.5 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 1 mL Nucleocuvette TM Cartridge
