Description
Product Overview The SF Cell Line 4D-Nucleofector® LV Kit XL* is suited for larger scale transfection of cell lines in the LV Nucleocuvette® Cartridge format when working with the 4D-Nucleofector® LV Unit. With the LV Nucleocuvette® Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation. This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal Nucleofector® Solution: HEK293 suspension clones CHO-S suspension clones K-562 U-937 The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. *Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-Nucleofector® System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support. Request More Info
Benefits Closed Nucleofection of up to 10 9 cell lines Conditions transferable from existing smaller scale protocols Automatic processing via tubing system Transfection of up to 20 mL reaction volume in less than 10 minutes Applications For large scale transfection of up to 1 x 10 9 cells Transient production of potential therapeutic proteins or antibodies for construct screening Generation of large numbers of transiently modified cell lines for cell-based assays
Storage and Content 5 x 22.5 mL SF Cell Line Nucleofector® Solution 5 x 5 mL Supplement 1 5 x LV Nucleocuvette® Cartridge 10 x 4D-Nucleofector® LV Reservoir
