Description
Product Overview Nucleocuvette® Cartridges PRO are the specific reaction vessels for the 4D-Nucleofector® LV Unit PRO in which the electroporation process takes place. They are used in combination with P3 Primary Cell Nucleofector® LV Solution Sets and 4D-Nucleofector® LV Reservoirs . The flow-through version, the LV Nucleocuvette® Cartridge PRO, enables processing of up to 1 billion cells in a sample volume of up to 20 mL. The cell suspension mixed with respective cargo (DNA, mRNA, RNP) is automatically transferred from an input reservoir into the cartridge and the transfected sample fraction is then transferred into a collection reservoir. Reservoirs may be the dedicated 4D-Nucleofector® LV Reservoirs or suitable bags. Before using the flow-through cartridge, the application should be established in the small-scale Nucleofection® Vessels on the 4D-Nucleofector® X Unit and then first scaled up to the fixed-volume cartridge of the 4D-Nucleofector® LV Unit PRO. Sterile welding : The PVC tubing allows for sterile welding to a TheraPEAK® Nucleofector® LV Reservoir or a bag. Alternatively sterile connections can be established manually via a Spinning Spiros connector. The GMP-grade counterpart, TheraPEAK® LV Nucleocuvette® Cartridge PRO, is planned to become available in Q4-2025. Disclaimer : Nucleofector® Products are for research use only and not intended for in vivo and/or diagnostic purposes. Request More Info
Benefits Single-use consumables Up to 20 mL processing volume Easily scalable to 1 billion cells Automatic filling via 4D-Nucleofector® LV Reservoirs or bags Connection to upstream and downstream equipment via Luer connectors (for research and early process development) or sterile welding (for late process development) Sterilized by ethylene oxide Applications Genome editing of large cell numbers for transient (mRNA, DNA) or stable (CRISPR-based knock-out or knock-in, transposon-based integration) Supporting use of 4D-Nucleofector® LV Unit PRO for research and process development
Storage and Content 1 LV Nucleocuvette® Cartridge PRO, weldable tubing
