Description
Product Overview The P3 Primary Cell 96-well Nucleofector® Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector® 96-well Unit. This kit has been determined to be optimal for transfecting following cell types: B cell (human) CD34+ cell (human) Chondrocyte (human) DC (human) DC (mouse), mature ES (human), H9 ES (mouse) Hepatocyte (human) HMEC (human) Keratinocyte (human) Macrophage (human) Monocyte (human) Neuron (rat), brain Hi/Cx NHBE (human) RPE (human) T cell (human), stim. T cell (mouse), unstim. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. The kit is available in different variants for either 1 x 96 or 10 x 96 reactions. Request More Info
Benefits High transfection efficiencies for primary cells Compatible with liquid handling systems for automated high-throughput electroporation Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System Applications Transfection of low cell numbers in the range of 5 x 10 4 to 1 x 10 6 cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content 2.25 mL P3 Primary Cell Nucleofector® Solution 0.5 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μL in 10 mM Tris pH 8.0) 1 x 96-well Nucleocuvette® Plate (20 μL)
