Description
Product Overview The SE Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit. This kit has been determined to be optimal for transfecting following cell types: 3T3-L1, diff. , 3T3-L1, undiff. A-10, A7r5 BHK-21, BJ C2C12 , CaCo-2 , Calu-3 , Capan-1, Cos-7 DU145 GH3 HCT116 , HeLa , HeLa S3 IMR-90 Jurkat (DSMZ), Jurkat E6.1 KG-1a, L6 MCF7 , MDA-MB-231 , MDA-MB-468, MDCK , MDCK II, MEG-01 , MG-63 , MRC-5 NCTC clone 929 Panc-1 T/G-HA VSMC, T-47D , T84 U2OS , U-87 MG WI-38 The respective cell type-specific Optimized Protocols are available available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. The kit is available in different variants for either 12 or 24 reactions. Request More Info
Benefits High transfection efficiencies in difficult-to-transfect cell lines Conditions are transferable between 4D Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System Applications Transfection of low cell numbers in the range of 5 × 10 4 to 1 × 10 6 cells Medium throughput applications Optimization of transfection conditions e.g. substrate titrations
Storage and Content 1 x 0.675 mL SE Cell Line Nucleofector® Solution 1 x 0.15 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 16-well Nucleocuvette® Strips (20 μL)
