Description
Product Overview The P3 Primary Cell 4D-Nucleofector® X Kit L is one of our five 4D-Nucleofector® Kits suited for transfecting primary cells in the 100 µL Nucleocuvette® Vessel format when working with the 4D-Nucleofector® X Unit. This kit has been determined to be optimal for transfecting following cell types: B cell (human) CD34+ cell (human) Chondrocyte (human) DC (human) DC (mouse), mature ES (human), H9 ES (mouse) Hepatocyte (human) HMEC (human) Keratinocyte (human) Macrophage (human) Monocyte (human) Neuron (rat) , brain Hi/Cx NHBE (human) RPE (human) T cell (human), stim. T cell (human), unstim. T cell (mouse), unstim. The respective cell type-specific Optimized Protocols are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. The kit is available in different variants for either 12 or 24 reactions. Request More Info
Benefits High transfection efficiencies in for primary cells Cells maintain functionality post transfection Conditions are transferable between 4D Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System Applications Transfection of cell numbers in the range of 2 × 10 5 to 2 × 10 7 cells Low throughput applications
Storage and Content 2 x 0.675 mL P3 Primary Cell Nucleofector® Solution 2 x 0.15 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 12 x 100 µL Nucleocuvette® Vessels 12 x Single use pipettes
