Description
Product Overview The P3 Primary Cell 4D-Nucleofector TM X Kit L is one of our five 4D-Nucleofector TM Kits suited for transfecting primary cells in the 100 µL Nucleocuvette TM Vessel format when working with the 4D-Nucleofector TM X Unit. This kit has been determined to be optimal for transfecting following cell types: ' B cell (human) CD34+ cell (human) Chondrocyte (human) DC (human) DC (mouse), mature ES (human), H9 ES (mouse) Hepatocyte (human) HMEC (human) Keratinocyte (human) Macrophage (human) Monocyte (human) Neuron (rat) , brain Hi/Cx NHBE (human) RPE (human) T cell (human), stim. T cell (human), unstim. T cell (mouse), unstim. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. The kit is available in different variants for either 12 or 24 reactions. Request More Info
Benefits High transfection efficiencies for primary cells Cells maintain functionality post transfection Conditions are transferable between 4D-Nucleofector TM System, 96-well Shuttle TM System and 384-well Nucleofector TM System Applications Transfection of cell numbers in the range of 2 x 10 5 to 2 x 10 7 cells Low throughput applications
Storage and Content 2.25 mL P3 Primary Cell Nucleofector TM Solution 0.5 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 24 x 100 µL Nucleocuvette TM Vessel 24 x Single use pipettes
