Description
Product Overview The Primary Cell Optimization 4D-Nucleofector® X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit. With the unique capability of the 4D-Nucleofector® X Unit to address individual wells of a 16-well Nucleocuvette® Strip with different programs, primary cell optimizations are easily performed within one experiment. Complete Optimization: Using five 16-well Nucleocuvette® Strips and our pmaxGFP TM Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette® Strip can be used for optional fine tuning or performing the first experiment with the selected conditions. Cell Group-Specific Optimization using Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs. Basic Protocols are available for following cell groups: Endothelial cells (mammalian) Epithelial cells (mammalian) Fibroblasts (mammalian) Glial cells (mammalian) Neurons (mammalian) Smooth muscle cells (mammalian) Stem cells (human) Before doing a complete or cell group-specific optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data. Request More Info
Benefits Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment Optimal Nucleofection conditions determined in the 16-well Nucleocuvette® Strips are transferable to the 100 μL single Nucleocuvette® in the 4D-Nucleofector® X Unit Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System Applications Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit
Storage and Content 0.675 mL Nucleofector® Solution P1 0.675 mL Nucleofector® Solution P2 0.675 mL Nucleofector® Solution P3 0.675 mL Nucleofector® Solution P4 0.675 mL Nucleofector® Solution P5 4 x 0.15 mL Supplement 1 1 x 0.15 mL Supplement 3 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 6 x 20 µL 16-well Nucleocuvette® Strips
