Product Overview The SF Cell Line 384-well Nucleofector® Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT Nucleofector® System. This kit has been determined to be optimal for transfecting following cell types: 293-F (FreeStyle), 32D A-20, A2058, A375, A431, A549, AGS, ARPE-19 BetaTC/6, BxPC-3 C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1 EL4 FDC-P1 H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C IMR-32 J774A.1 K-562 KG-1 L-428, LNCaP MDA-MB-453, MDBK, Molt-4, MV-4-11 Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0 P19, P815, PC-12, PC-3 Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226 S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480 T2, T84, TF-1 U266B1, U-937 Vero The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. The kit is available in different variants for either 2 x 384 or 10 x 384 reactions. Request More Info
Benefits High transfection efficiencies in difficult-to-transfect cell lines Cells maintain functionality post transfection Seamless integration into automated liquid handling environments Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System Applications Transfection of low cell numbers down to 2 x 10 4 cell lines High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content 22.5 mL SF Cell Line Nucleofector® Solution 5 mL Supplement 1 50 µg pmaxGFP TM Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 384-well Nucleocuvette® Plate (20 μL)
