Description
Product Overview The Primary Cell Optimization 384-well Nucleofector® Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector® System. Complete Optimization: Different conditions can easily be tested within one experiment using a 384-well Nucleocuvette® Plate and our pmaxGFP TM Vector as positive control. Our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our scientific support team. Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs. Basic Protocols are available for following cell groups: Endothelial cells (mammalian) Epithelial cells (mammalian) Fibroblasts (mammalian) Glial cells (mammalian) Neurons (mammalian) Smooth muscle cells (mammalian) Stem cells (human) Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data. Request More Info
Benefits High transfection efficiencies for primary cells Automated high-throughput electroporation Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment Optimal Nucleofection conditions determined on the 384-well HT Nucleofector® System are transferable to the 4D-Nucleofector® System or the 96-well Shuttle® Add-On Applications Transfection of low cell numbers in the range of 2 x 104 to 1 x 10 6 cells Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector TM System
Storage and Content 2.25 mL Nucleofector® Solution P1 2.25 mL Nucleofector® Solution P2 2.25 mL Nucleofector® Solution P3 2.25 mL Nucleofector® Solution P4 2.25 mL Nucleofector® Solution P5 4 x 0.15 mL Supplement 1 1 x 0.15 mL Supplement 3 150 µg pmaxGFP TM Vector (1 μg/μL in 10 mM Tris pH 8.0) 1 x 384-well Nucleocuvette® Plate
