Description
Product Overview The Cell Line Optimization Nucleofector® Kit is the ideal tool to determine the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the Nucleofector® 2b System (or older generations I or II). It enables you to conveniently determine the optimal Nucleofection condition of your cell line of interest within one experiment. The kit contains two different Cell Line Nucleofector® Solutions, V and L, each of which should be tested in combination with seven different Nucleofector® Programs. It is suitable for 18 optimization reactions. Fine-tuning for optimal results can then be performed together with our Scientific Support Team . Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data. Request More Info
Benefits Efficient transfection of virtually any difficult-to-transfect cell line Simple and rapid optimization completed within just one experiment Applications Optimization of Nucleofection conditions of virtually any difficult-to-transfect cell line with the Nucleofector® II/2b Device
Storage and Content 0.9 mL Cell Line Nucleofector® Solution L 0.9 mL Cell Line Nucleofector® Solution V 2 x 0.2 mL Supplement 1 2 x 30 µg pmaxGFP TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 20 Aluminum cuvette (100 µL) 20 Single use pipettes
