Lonza, VPA-1002, Human T Cell Nucleofector® Kit

Product Overview The Nucleofector® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector® Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated). The cell type-specific Optimized Protocol is available in our knowledge database . In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. Request More Info
Benefits High transfection efficiencies for primary cells Cells maintain functionality post transfection Applications Transfection of cell numbers in the range of 2 × 10 5 to 2 × 10 7 cells Low-throughput transfection in single cuvette format
Storage and Content 2.25 mL Nucleofector® Solution for Human T Cells 0.5 mL Supplement 1 30 µg pmaxGFP TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 25 Aluminum cuvette (100 µL), 25 Single use pipettes