Lonza, VPA-1003, Human CD34+ Cell Nucleofector® Kit

Product Overview The Nucleofector® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector® Kit is the optimal kit for efficient transfectiion of primary human hematopoietic stem cells (CD34+) from cord blood or bone marrow. The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database . In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type. Request More Info
Benefits High transfection efficiencies for primary cells Cells maintain functionality post transfection Applications Transfection of cell numbers in the range of 2 × 10 5 to 2 × 10 7 cells Low-throughput transfection in single cuvette format
Storage and Content 2.25 mL Nucleofector® Solution for Human CD34+ Cells 0.5 mL Supplement 1 30 µg pmaxGFP TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 25 Aluminum cuvette (100 µL) 25 Single use pipettes