Description
Product Overview The Nucleofector® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different MEF clones, we have developed two Nucleofector® Kits (1 and 2) and an easy-to-use Nucleofector® Starter Kit. The Mouse Embryonic Fibroblast Nucleofector TM Starter Kit is used to determine which of the two Nucleofector® Kits is best suited for transfecting a specific primary mouse embyonic fibroblast (MEF) clone in Nucleofector® 2b System. The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database . In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type. Request More Info
Benefits High transfection efficiencies for primary cells Cells maintain functionality post transfection Applications Transfection of cell numbers in the range of 2 × 10 5 to 2 × 10 7 cells Low-throughput transfection in single cuvette format
Storage and Content 0.45 mL MEF 1 Nucleofector® Solution 0.45 mL MEF 2 Nucleofector® Solution 2 x 0.1 mL Supplement 1 30 µg pmaxGFP TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 10 Aluminum cuvette (100 µL) 10 Single use pipettes
