Description
Product Overview In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector® Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector® 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector TM Programs is tested in combination with the Basic Nucleofector® Solution for smooth muscle cells. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database . In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. Request More Info
Benefits Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 95% Viabilities up to 96% Applications Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Storage and Content 2.25 mL Nucleofector® Solution forPrimary Mammaliean Smooth Muscle Cells 0.5 mL Supplement 1 30 µg pmaxGFP TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 25 Aluminum cuvette (100 µL) 25 Single use pipettes
