Merck, 06011801-DNA-5UG, 1777N Rpmet

Biochem/physiol Actions
Human embryonal carcinoma
This cell line was established from a retro-peritoneal metastasis of a non seminomatous germ cell carcinoma.
Preparation Note
DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO₂; 37°C. Cells need to be maintained at a high cell density.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].