| NACRES | NA.74 |
|---|---|
| UNSPSC Code | 41106213 |
| agency | according to ISO 7899-2 |
| sterility | non-sterile |
| form | granular |
| packaging | pkg of 500 g |
| manufacturer/tradename | GranuCult® plus |
| storage condition | protect from light (Keep tightly closed and dry) |
| technique(s) | microbe id | specific enzyme detection: suitable |
| color | yellow |
| pH | 6.9-7.3 (25 °C, 54.85 g/L in H2O, after autoclaving) |
| solubility | 54.85 g/L |
| application(s) | food and beveragesmicrobiology |
| storage temp. | 15-25°C |
| suitability | Enterococcus spp. |
| Quality Level | 200 |
Answer:Confirmation and presumptive identification of intestinal enterococci in water testing.
Answer:Esculin hydrolysis produces esculetin, which reacts with ferric ions to form a dark complex.
Answer:It is a 500 g bottle of non-sterile granulated medium.
Bile Aesculin Azide Agar is designed for selective culture and presumptive identification of intestinal enterococci during microbiological water analysis. The GranuCult® plus dehydrated medium is supplied as 500 g of granules and is suitable for the confirmation stage of an ISO 7899-2 membrane-filtration workflow. Bile salts help suppress susceptible accompanying organisms, and sodium azide suppresses Gram-negative microbial flora. Enterococci hydrolyze esculin to esculetin; the resulting reaction with ferric ions produces a dark olive-green to black complex around typical colonies. This reaction, together with bile tolerance, supports identification of the presumptive colonies recovered from a primary selective membrane agar. The recommended preparation is approximately 54.85 g per liter of water, with a final pH of 6.9–7.3 after autoclaving under the stated conditions. The medium is supplied non-sterile and requires preparation before use. Store the tightly closed container dry, protected from light, at 15–25 °C. It is a confirmation medium rather than the TTC-containing primary enumeration medium.
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