Merck, 10065-50G, α-Amylase from Aspergillus oryzae

General description
Microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. α-Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration).
Application
α-Amylase has been used:
as a control enzyme in agar plate-based and carboxymethylcellulose-based clearing assays to screen cellulase activity
for the hydrolysis of starch to explore the the role of wheat starch in frozen dough
to inhibit Staphylococcus aureus biofilms

Biochem/physiol Actions
Aspergillus oryzae α-amylase (Ao α-amylase) enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins.
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).
For the determination of fats in food; Application in (selective) hydrolysis/condensation of glycosidic bonds.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer