Merck, 10107824001, NADPH

General description
Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm
Absorbance maxima: 260 nm and 340 nm
Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4
pH value in water: 8.0 ± 1.0 (10 mg/ml)

Amorphous powder bottled under nitrogen.
Application
NADPH-RO has been used for the determination of thioredoxin reductase activity.
Biochem/physiol Actions
NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase.
Preparation Note
Storage conditions (working solution): 2 to 8 °C
10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C.
Analysis Note
Purity: =97% β-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm)
Organic solvents: =3% (GC)

Absorbance coefficients: 15.0 x 10³ l x mol⁻¹ x cm⁻¹ at 260 nm, 6.3 x 10³ l x mol⁻¹ x cm⁻¹ at 340 nm
Other Notes
For life science research only. Not for use in diagnostic procedures.
Packaged based on solid weight.