Merck, 10127990001, NAD

General description
Nicotinamide adenine dinucleotide (NAD) is a cofactor containing an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. It is ubiquitously found. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.
Application
NAD has been used in boiled-blood agar plate as a supplement for the growth of Actinobacillus pleuropneumoniae colony.
Biochem/physiol Actions
Electron acceptor
Nicotinamide adenine dinucleotide (NAD) acts as an electron carrier and significantly participates in enzyme-catalyzed oxido-reduction processes and many genetic processes. NAD cycles between the oxidized (NAD⁺) and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth. This cofactor is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.
Packaging
Packaged by solid weight.
Preparation Note
Storage conditions (working solution): An aqueous solution in a concentration of 10 mg/ml (pH 2.5 to 3) maintains > 99% of its NAD content after 8 weeks of storage at 2 to 8 °C.
The stability of an aqueous solution in a concentration of 40 mg/ml is not expected to differ from that of a solution in a concentration of 10 mg/ml.

An aqueous solution in a concentration of 50 mg/ml (pH 2.0) maintains 97% of its NAD content after 2 days, 94% after 6 days and 91% after 10 days of storage at 2 to 8 °C and about 83% after 10 days of storage at 33 °C.

An aqueous solution in a concentration of 1.38 mg/ml shows a decrease of NAD content of 4% after 8 weeks of storage at 2 to 8 °C, stored at -15 to -25 °C the stability is expected to be > 6 months. A solution of NAD in triethanolamine buffer (pH 5.0) maintians 90% of its NAD content after
10 days of storage at 2 to 8 °C and 80% after 10 days of storage at 33 °C.
Analysis Note
β-Nicotinamide adenine dinucleotide hydrate cofactor (NAD) has been used in an experimental protocol, done in order to mimic the citric acid cycle on an carbon electrode, in order to increase biofuel cell energy density.
Absence of contaminants: LDH inhibitors are not detectable.
Organic solvents: <0.5% (GC)
Absorbance coefficients: 17.6 x 10³ l x mol⁻¹ x cm⁻¹ at 260 nm, 5.9 x 10³ l x mol⁻¹ x cm⁻¹ at 333 nm (CN complex)
Other Notes
For life science research only. Not for use in diagnostic procedures.
This is the common form of NAD.