Merck, 1072285000, Buffered Peptone Water

Name: Buffered Peptone Water
Brand: Merck
SKU: 1072285000

Size:5 kg, BPW, Buffered Peptone Solution, GranuCult® BPW, Peptone Water (buffered), Peptone Water, buffered, Peptone Water, phosphate-buffered, Tryptone Phosphate Water, GranuCult® prime, ISO 19250, ISO 6579-1, ISO 21528-1, ISO 22964, FDA-BAM, EP, granular, pkg of 0.5-25 kg

Catalog Number: 1072285000

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Category

Dehydrated Culture Media

Pack Size5 kg

TagsIndustrial Microbiology Microbial Culture Media

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Buffered Peptone Water is rich in nutrients and produces high resuscitation rates for sub lethally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone including enzymatic digest of casein acts as a source of carbon, nitrogen, vitamins and minerals whilst sodium chloride maintains the osmotic balance.
GranuCult® is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
Application
Buffered Peptone Water is used in the testing of food and water samples. This product is suitable for the preliminary non selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae such as Salmonella and Cronobacter, from food, animal feed, water, and other materials. The product is also suitable as a diluent for general use.
Preparation Note
Store at +15 °C to +25 °C, dry and tightly closed.
Do not use clumped or discolored medium.
Protect from UV light (including sunlight).
For in vitro use only.
According to EN ISO/FDIS 6579-1, the self-prepared medium can be stored in closed containers at +2 °C to +8 °C in the dark and protected against evaporation for up to six months.

Analysis Note
Appearance (clearness): clear
Appearance (colour): yellowish
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133 and the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Salmonella Abony NCTC 6017): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Salmonella Abony NCTC 6017): good to very good
Incubation:
18 ± 2 hours at 37 ± 1 °C
S.abony 18 - 24 hours at 30 - 35 °C
Other Notes
Replaces: 1072285007; 1.07228
The prepared medium is clear and yellowish.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture medi. depending on your needs. The designations basi. plu. or prime are added to indicate the quality control leve. from basic quality control to standard QC plus to prime for full regulatory compliance.

Specifications

General

NACRES	NA.74
UNSPSC Code	41106213
agency	APHA
sterility	non-sterile
form	granular
packaging	pkg of 0.5-25 kg
manufacturer/tradename	GranuCult® prime
technique(s)	microbiological culture: suitable
pH	7.2 (37 °C, 25 g/L in H2O, after autoclaving)
solubility	25.5 g/L
bulk density	800 kg/m3
web metadata keyword.default	microbialanalysismicrobialanalysis
application(s)	food and beveragesmicrobiologypathogen testing
storage temp.	15-25°C
suitability	Cronobacter spp.
Quality Level	200

FAQs

Question: What quantity is supplied?

Answer:A 5 kg pack of dehydrated granulated medium.

Question: Is Buffered Peptone Water selective?

Answer:No. It is used for nonselective preliminary enrichment.

Question: What preparation concentration is given?

Answer:Approximately 25.5 g/L.

Application Note

Merck GranuCult® prime Buffered Peptone Water is a nonselective enrichment medium for microbiological testing of food, animal feed, water, and related samples. This product provides 5 kg of dehydrated granules intended for preparation of buffered peptone enrichment broth. Nutrients support recovery and resuscitation of stressed bacteria, while phosphate buffering helps maintain a suitable pH during early incubation. Applications include preliminary enrichment of Salmonella, Cronobacter, and other Enterobacteriaceae before subsequent selective detection steps. The stated preparation is approximately 25.5 g/L, with an expected pH around 7.2 under specified test conditions. GranuCult prime medium is associated with relevant ISO and FDA-BAM microbiological workflows. The product is a bulk dry medium, not a ready-to-use liquid broth or selective isolation agar.

Ordering

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