Merck, 10771-500ML, Histopaque®-1077

General description
Histopaque-1077 is a sterile, endotoxin tested, ready-to-use medium. The Histopaque composition includes polysucrose and sodium diatrizoate, adjusted to a relative density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood or bone marrow.
Application
Histopaque-1077 has been used for:human lymphocyte antigen (HLA) typing
initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes.
preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
isolation of human lymphocytes
isolation of breast cancer circulating tumor cells (CTCs) from the whole blood
Histopaque-1077 is used in conjunction with Histopaque-1119 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood

Biochem/physiol Actions
Anticoagulated blood is layered onto Histopaque-1077. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment. Granulocytes become slightly hypertonic, which increases their sedimentation rate, resulting in pelleting at the bottom of the centrifuge tube. Lymphocytes and other mononuclear cells remain at the plasma/ Histopaque-1077 interface.
Features and Benefits
Histopaque®-1077 offers numerous advantages, such as:
Optimal recovery of viable cells
Permits selective separation of blood cell types
Prevents cell distortion
Preserves cell viability
Minimal extraneous cell interference
Reproducible performance from batch to batch
Sterile-filtered and endotoxin-tested
Stable at 2-8°C for at least 2 years when protected from light

Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany