Merck, 10831-100ML, Histopaque®-1083

General description
Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.
Application
Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.
Biochem/physiol Actions
Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.
Features and Benefits
Histopaque®-1083 offers numerous advantages, such as:

Optimal recovery of viable cells
Permits selective separation of blood cell types
Prevents cell distortion
Preserves cell viability
Minimal extraneous cell interference
Reproducible performance from batch to batch
Sterile-filtered and endotoxin-tested
Stable at 2-8°C for at least 2 years when protected from light

Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany