Merck, 1112780500, M FC agar

General description
m-FC Agar is a selective membrane filtration medium suitable for the detection of fecal coliforms. The fecal coliforms derived from the intestinal tract of warm-blooded animals are differentiated from the other environmental coliforms due to their ability to grow at higher temperatures. The medium is supplemented with rosolic acid and incubated at 44.5 °C ± 0.2 °C for 24 h. Peptone and yeast extract, which serve as nutritious sources, and bile salts are added to inhibit accompanying Gram-positive flora. Lactose can be fermented by fecal coliforms at elevated temperatures to form blue colonies on the prepared medium (agar base with rosolic acid), whereas other organisms form grey colonies.
Application
m-FC Agar is used for the cultivation and enumeration of fecal coliforms by the membrane filtration technique.
Features and Benefits
GranuCult® offers superior granulated culture media
Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
Excellent wettability, solubility, and free-flowing properties
Convenient, with minimal component separation and clumping, even under warm or humid conditions
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note
Appearance (clearness): clear
Appearance (colour): blue to violet
pH-value (25 °C): 7.2 - 7.6
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good; colony colour blue to dark blue
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good; colony colour pink to red
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): no growth
Growth (Enterobacter aerogenes ATCC 13048): good; colony colour grey to grey-blue
Incubation: 24 hrs.; 44.5 °C; aerobic.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany