Merck, 138630-100G, 1-Chloro-2,4-dinitrobenzene

Application
1-Chloro-2,4-dinitrobenzene was used in determination of relative amounts of individual glutathione S-transferases subunits in human tissues by reverse-phase HPLC. It was used as contact sensitizer to study the antibacterial peptides hBD-2, -3 and -4 and LL-37 induced IL-18 protein release in human keratinocytes.
Biochem/physiol Actions
2,4-dinitrochlorobenzene induces atopic dermatitis in BALB/c mice model.
Preparation Note
The product is dissolved in ethanol when used as glutathione S-transferase substrate and then added to a buffer to form a final concentration of 1mM in phosphate buffer (8 mL of 0.2 M KH₂PO₄ and 28 mL of 0.1 M Na₂HPO₄ per 100 mL of solution, adjusted to pH 6.5) and 4% (v/v) ethanol.