Merck, 178497-10MG, Apoptosis Activator VII, Apoptozole

General description
A cell-permeable imidazole compound that directly binds anti-apoptotic chaperone Hsc70 and its inducible homologue Hsp70 (Kd = 210 and 140 nM, respectively). Apoptozole is shown to effectively inhibit the proliferation of SK-OV-3, HCT-15, and A549 cancer cell lines (IC₅₀ = 220, 250 and 130 nM, respectively) and induce 90% apoptotic cell death in P19 embryonic carcinoma and A549 lung cancer cultures after 12 h drug treatment (1 µM). Shown to suppress ubiquitination of mutant CFTR and promote membrane trafficking, and restore chloride channel activity.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Cho, H.J., et al. 2012. J. Am. Chem. Soc.133, 20267.
Williams, D.R., et al. 2008. Angew. Chem. Int. Ed.47, 7466.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)