Merck, 204882-50UG, Complement C2, Human

General description
Native, human C2 complement component. Single chain glycoprotein present in normal human serum at concentration of 20 µg/ml. Classical pathway activation results in assembly of the C1,C4b complex on target surfaces. Subsequent to binding of the C4b component to this complex, C2 is cleaved by the activated C1s subcomponent of C1 into C2a, the 63 kDa C-terminal fragment, and C2b, the 30 kDa N-terminal fragment. The smaller C2b fragment is released while the larger C2a fragment remains associated with the C1,C4b complex to form the C4b,C2a classical pathway C3 convertase enzyme. C2 concentration is based on functional activity measurements relative to a normal human serum standard pool.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, pH 6.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Attwood, J.T., et al. 2001. Acta Paediatr.90, 99.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)