Merck, 208783-1MG, Calmodulin, Porcine Brain

General description
Calmodulin (CaM) purified from porcine brain. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates processes like inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for applications requiring active pure calmodulin.
Physical form
Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Massom, L., et al. 1990. Biochemistry29, 671.
One unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3′5′-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca²⁺ in a 3 ml reaction volume at 30°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)