Merck, 219466-50KU, CELLULYSIN™ Cellulase, Trichoderma viride

General description
Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.
Application
CELLULYSIN™ Cellulase, Trichoderma viride has been used:
to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
in the enzyme mix used for in situ hybridization to plant chromosomes
in the enzyme solution for the transformation of Phytophthora species
to isolate intact cells and protoplasts from plant tissues

Biochem/physiol Actions
Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.
Other Notes
One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.
Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)