Merck, 234400-M, Complement, Rabbit Serum

General description
Complement, Rabbit Serum is a native, rabbit serum complement. Tested for suitability for HLA-ABC serology. Total lympholytic complement activity determined under conditions of NIH two-step cytotoxicity test that uses defined HLA isoantisera. Activity present at a titer of at least 1:4. No intrinsic cytotoxicity against human peripheral blood lymphocytes. Intended for micro and macro lymphocyte cytotoxicity testing with human or animal sera. Also useful as an active source of complement for use with hybridoma antibodies, alloantisera, and related applications in which a non-cytotoxic source is required.
Application
Complement, rabbit serum has been used:
in complement killing of fibroblasts for the isolation of Schwann cells (SCs)
as a component of Dulbecco′s modified Eagle medium (DMEM) for the purification of SC cells
to eliminate fibroblasts for the SC isolation

Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for 24 h at 4°C or for up to 1 month at -20°C.
Reconstitute with 5 ml of dH₂O.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)