Merck, 3014-A, Transcreener® GDP FI Assay

General description
The Transcreener® GDP FI Assay is a competitive immunoassay for GDP with a red fluorescent intensity (FI) readout. Designed specifically for HTS and featuring a single addition mix-and-read format and room temperature reagent stability, the Transcreener® GDP FI Assay is compatible with plate readers typically found in research labs as well as those used in HTS core facilities. The 3014-A kit is formatted for 96-well plates while the 3014-1K and 3014- 10K kits are for 384-well and higher densities.

Able to accommodate GTP concentrations ranging from 0.1 to 1,000 μM, the Transcreener® GDP FI Assay provides excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10% or less). Because the proprietary antibody used is highly selective for GDP, the assay can be used with any enzyme that converts GTP to GDP, including small G proteins. (Note that many commercially available GTPase preparations contain GDP as a stabilizer, which must be removed or diluted prior to use with this assay so as not to contribute to background signal.

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Physical form
Kit with buffered aqueous solutions
Preparation Note
3014-1K = 1,000 assay, 384-well
3014-10K = 10,000 assay, 384-well
3014-A = 200 assay, 96-well
Legal Information
Transcreener is a registered trademark of BellBrook Labs