Description
General description
A water-soluble nonionic detergent. Studies on rhodopsin have shown that alkyl glycosides with longer alkyl chains are better at preserving the protein′s properties. In addition, dodecyl maltoside′s longer hydrophilic group confers higher solubility in water. This is in contrast to undecyl and dodecyl glucosides, which have low solubility in water. Due to its low CMC, dodecyl maltoside is not easily removed by dialysis. The half time for dialysis is 20 h. This detergent is milder than nonyl glucoside. Of several glycosides tested, dodecyl maltoside was found to be the most useful for the preparation of active cytochrome oxidase from Neurospora. In octyl glucoside, cytochrome oxidase dissociated into its subunits and became inactive. Similarly, beef heart oxidase when equilibrated in octyl glucoside formed a mixture of polymer, dimer, and lower molecular weight forms and underwent denaturation. In contrast, the enzyme, when equilibrated in dodecyl maltoside, was found to exist as a uniform species of 30 kDa, which is consistent with an active dimeric form. Dodecyl maltoside has a critical micelle concentration (CMC) of 100-600 µM and a micellar weight of 70,000. Aggregation number: 98.
Absorbance (10%, H2O, 260 nm): n-octyl-β-D-glucopyranoside; however, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution. Useful in membrane-protein solubilization studies. Aggregation number: 98.
Application
Maximizing hydrophobic peptide recovery in proteomics and antibody development using a mass spectrometry compatible surfactant.: The article explores the use of n-Dodecyl β-D-maltoside to maximize hydrophobic peptide recovery in proteomics. The findings highlight the surfactant′s compatibility with mass spectrometry, improving peptide analysis and antibody development. (Nie et al., 2022).
Sickle-like Inertial Microfluidic System for Online Rare Cell Separation and Tandem Label-Free Quantitative Proteomics (Orcs-Proteomics).: This research introduces an inertial microfluidic system utilizing n-Dodecyl β-D-maltoside for rare cell separation and proteomics. The study underscores the surfactant′s role in enhancing cell recovery and proteomic analysis. (Wang et al., 2022).
Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics.: This paper presents a method for single-cell proteomics using n-Dodecyl β-D-maltoside. The surfactant assists in efficient sample preparation, facilitating high-resolution proteomic analysis. (Tsai et al., 2021).
Preparation Note
Following reconstitution, refrigerate (4°C).
Stock solutions are stable for up to 3 months at 4°C.
Other Notes
Witzmann, F., et al. 1991. Electrophoresis12, 687.
Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem.171, 330.
Rosevear, P., et al. 1980. Biochemistry19, 4108.
Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids23, 321.
Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta425, 46.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
