Description
General description
Fibronectin is a glycoprotein belonging to the extracellular matrix (ECM) protein family. Fibronectin contains two polypeptides (α and β chains) linked by two disulfide bonds near the carboxyl termini to form a dimer.Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Application
The human plasma fibronectin has been used for coating surfaces such as poly-l-lysine (PLL)-coated nanochips, polyacrylamide (PAAm) hydrogel substrates, glass slides and more, providing a substrate for the adhesion and culture of cells in various experimental setups.
Biochem/physiol Actions
Human fibronectin protein plays crucial roles in various cellular behaviors such as cell attachment, migration, embryogenesis, wound healing, tissue integrity, and malignant processes. One of the key features enabling fibronectin to support cell adhesion is the presence of the Arg-Gly-Asp (RGD) tripeptide. It has been extensively studied in areas like fibronectin coating on polystyrene (PS) and poly(lactic-co-glycolic acid) (PLGA) surfaces. Additionally, fibronectin has been the subject of significant research regarding its involvement in regulating processes such as differentiation-to-growth switching, tubulin monomer growth, cell cycle status, cell spreading, and motility.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, pH 7.3.
Preparation Note
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Contaminants: none detected by IEP with antiserum to fibronectin or normal human serum
single major band by SDS-PAGE
Other Notes
Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol.59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett.64, 152.
Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem.283, 2297.
Brown, S., et al. 2002. Nature418, 200.
Kim, M., FEBS Letters581 1917.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Toxicity: Standard Handling (A)
