Description
General description
A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange. Shown to cause a rapid activation of ERK1/2 (EC₅₀ ~ 2.5 - 5 µM) in rat arterial smooth muscle and Rat2 cells. Also promotes the phosphorylation of JNK and p38 MAP kinase, phospholipase C and causes Ca²⁺ release from internal stores. Negative control is also available (Cat. No. 371820).
Biochem/physiol Actions
Cell permeable: yes
EC50 ~ 2.5 - 5 µM in activation of ERK1/2 in rat arterial smooth muscle and Rat2 cells
Primary Target
N-myristoylated G-protein βγ selective binding
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C
Other Notes
Myr-N-Ser-Ile-Arg-Lys-Ala-Leu-Asn-Ile-Leu-Gly-Tyr-Pro-Asp-Tyr-Asp-OH
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
