Merck, 399275-1MG, Nω-Hydroxy-nor-L-arginine, Diacetate Salt

General description
A potent, selective, competitive, and high affinity inhibitor of arginase. Shown to inhibit arginase from rat liver (IC₅₀ = 2 µM) and mouse macrophages (IC₅₀ = 50 µM). Shown to specifically interact with the manganese-cluster of the enzyme active site. Does not function as a substrate or as an inhibitor for NOS. Hence, nor-NOHA may be a useful tool to study the interplays between arginase and NOS.
Biochem/physiol Actions
Primary Target
arginase from rat liver
Secondary Target
mouse macrophages (IC₅₀ = 50 µM)
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Gobert, A.P., et al. 2000. Infect. Immun. 68, 4653.
Meurs, H., et al. 2000. Br. J. Pharmacol. 130, 1793.
Moali, C., et al. 2000. Biochemistry 39, 8208.
Tenu, J.P. et al. 1999. Nitric Oxide 3, 427.
Moali, C., et al. 1998. Biochemistry 37, 10453.
Custot, J., et al. 1997. J. Am. Chem. Soc. 119, 4086.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)