Merck, 407700-5UG, Interleukin-10, Human, Recombinant, S. frugiperda

General description
Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Blocks lipopolysaccharide induction of MMP-2 and MMP-9. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Human IL-10 is also active on murine cell lines.
Biochem/physiol Actions
ED₅₀ = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.
Physical form
Carrier-free.
Preparation Note
Following reconstitution, aliquot and freeze -70°C. Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Reconstitute in sterile water or buffer to a final concentration of 0.1-1 mg/ml as stock solution. Further dilute in buffer such as PBS, 1% BSA.
Other Notes
Bachis, A., et al. 2001. J. Neurosci.21, 3104.
Fortunato, S.J., et al. 2001. Obstet. Gynecol.98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)