Merck, 420400-50UG, Anti-KDEL Mouse mAb (10C3)

General description
Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
Recognizes the Grp78 (BiP) and Grp94 proteins.
This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.
Immunogen
Rat
a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
Application
Immunoblotting (4 µg/ml, colorimetric)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (5 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
Other Notes
Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic (H)