Merck, 454850-2000U, MAP Kinase 2, Mouse, Recombinant, E. coli

General description
Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
Physical form
In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% Brij® 35 detergent, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)