Merck, 51120-25MG, Guanosine 5′-triphosphate sodium salt hydrate

• as a component of the internal solution for astrocyte patch-clamp to monitor astrocyte calcium activity from mouse amygdala slices.
• in a solution for channel current recording in cell lines using a voltage-clamp technique.
• to prepare internal recording solution for injectoporation to deliver genes to mechanosensory hair cells.
• promoting the formation of G interface-mediated homodimers of human septin during the analysis of its oligomerization state by analytical size exclusion chromatography (SEC).

Guanosine 5′-triphosphate sodium salt hydrate is a purine nucleotide supplied as a powder with an assay of at least 90% by HPLC. This configuration contains 25 mg. GTP participates in protein synthesis, G-protein signaling, microtubule assembly, and many other nucleotide-dependent biochemical reactions. The reagent is useful for enzymology, molecular biology, cell-signaling research, protein translation systems, and biochemical assay development. It dissolves in water at approximately 50 mg/mL to form a clear, colorless solution. The anhydrous free-acid molecular weight is 523.18 g/mol. The product is shipped cold and stored frozen at approximately −20 °C.