Description
General description
Native pepsin from porcine stomach mucosa. Secreted by the mucosal lining of the stomach. Active under acidic conditions. Catalyzes the hydrolysis of aminoacyl-proline to an amino acid and proline. Inhibitors include aliphatic alcohols, pepstatin A, and pH >6.0.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Bergmeyer, H.V. 1984. Methods of Enzymatic Analysis (3rd ed., vol. 5) p. 232, Academic Press, New York.
One unit is defined as the amount of enzyme that will digest hemoglobin substrates to TCA-soluble products, measured as a change in absorbance at 280 nm of 0.001 per min at 37°C, pH 2.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)