Merck, 524604-100U, Phosphatase, Alkaline, Human Placenta

General description
Native alkaline phosphatase from human placenta. Homodimeric enzyme that hydrolyzes phosphate esters of primary and secondary alcohols, amines, and phenols. Useful in the hydrolysis of 5′-terminal phosphates of DNA and RNA. Active site contains a reactive serine.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 50% glycerol, pH 7.6.
Preparation Note
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Fishman, W.H., et al. 1973. Anal. Biochem.51, 368.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 μmol pNPP per min at 37°C, pH 10.15.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)