Description
General description
Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.
Application
Phosphatase Inhibitor Cocktail Set III has been used:
as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
as a component in cell lysis buffer to extract total proteins for western blotting
Biochem/physiol Actions
Cell permeable: no
Primary Target
Serine/threonine and protein tyrosine phosphatases
Product does not compete with ATP.
Reversible: no
Features and Benefits
Ready-to-use formulations for convenient use
High-quality products for consistent and reproducible performance,
Flexible options with a range of cocktails targeting different categories of phosphatases
Physical form
In H₂O.
Preparation Note
For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Each vial contains 1 ml of aqueous solution with the following components:
50 mM Sodium Fluoride
10 mM β-Glycerophosphate (Cat. No. 35675)
10 mM Sodium Pyrophosphate Decahydrate
1 mM Sodium Orthovanadate
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Toxic (F)
