Description
General description
A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials.
Application
Phosphatase Inhibitor Cocktail Set V, 50X has been used:
as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
in lysis buffer to lyse cultured HUVECs for immunoprecipitation
in wash buffer to wash the protein complexes
as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity
Biochem/physiol Actions
Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.
Features and Benefits
Convenient: formulations are ready-to-use, making them easy to use.
Consistent: high-quality formulations ensure reliable and reproducible performance.
Flexible: the cocktails are designed to target different categories of phosphatases, offering versatility in experimentation.
Physical form
In H₂O.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C) for long-term storage. Dilute 1:50 just prior to use.
Other Notes
Each vial contains 1 ml of aqueous solution with the following components:
250 mM Sodium Fluoride
50 mM β-Glycerophosphate (Cat. No. 35675)
50 mM Sodium Pyrophosphate Decahydrate
50 mM Sodium Orthovanadate
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Toxic (F)
