Merck, 535142-1ML, Protease Inhibitor Cocktail Set I, Animal-Free

General description
A cocktail of five protease inhibitors for the inhibition of a broad range of proteases and esterases. Each vial, when reconstituted with 1 ml H2O, yields a 100X stock solution. When diluted to 1X the cocktail contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616371), 1 µM E-64 (Cat. No. 324890), 0.5 mM EDTA, Disodium Salt and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Available as 1 ml or as a set of 10 x 1 ml.
Application
Protease Inhibitor Cocktail Set I, Animal-Free has been used:
as a supplement in cell lysis buffer to extract proteins from non-small-cell lung carcinoma (NSCLC) cell lines
in NP-40 buffer to lyse the cells for sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting
as a component in lysis buffer to lyse transfected Huh7 cells and to wash the pellets to detect hepatitis B virus (HBV) C protein phosphorylation in the cell

Biochem/physiol Actions
Protease inhibitor cocktails and specific protease inhibitors are valuable resources for scientists involved in proteomic studies, such as protein expression, characterization, biomarker discovery, post-translational modification mapping, protein profiling, and quantitative protein measurement. By adding a protease inhibitor cocktail or specific inhibitor, the integrity of proteins is preserved for subsequent analysis and further sample characterization.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial in 1 ml H₂O to yield a 100X stock solution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)