Merck, 53702-250KU, Pronase® Protease, Streptomyces griseus

General description
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
Note: 1 KU = 1000 units.
Physical form
Lyophilized from a solution containing calcium acetate as a stabilizer.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
Other Notes
Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)