Merck, 537059-250MG, Propidium Iodide

General description
Extinction coefficient(493 nm): 5900 M ⁻¹cm⁻¹;(287 nm): 55,700 M ⁻¹cm⁻¹;
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M⁻¹cm⁻¹ (493 nm); 55,700 M⁻¹cm⁻¹ (287 nm); 13,800 M⁻¹cm⁻¹ (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
Biochem/physiol Actions
Product does not compete with ATP.
Reversible: no
Primary Target
Membrane impermeable DNA intercalator
Cell permeable: no
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
Other Notes
Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)