Merck, 539131-1VL, Protease Inhibitor Cocktail Set I

General description
Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.
Application
Protease Inhibitor Cocktail Set I have been used as a supplement in:
cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
sucrose solution to homogenize mice cortices for myelin isolation

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Biochem/physiol Actions
Inhibits a broad range of proteases and esterases.
Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.
Features and Benefits
Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
Ready for reconstitution

Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.
Other Notes
The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)